size-based separation method for viable ctcs enrichment Search Results


cellsievetm microfiltration assay  (Creatv MicroTech)
90
Creatv MicroTech cellsievetm microfiltration assay
Studies on the clinical relevance of programmed death ligand 1-positive (PD-L1 + ) circulating tumor cells (CTCs) in non-small cell lung cancer (NSCLC) .
Cellsievetm Microfiltration Assay, supplied by Creatv MicroTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cellsievetm microfiltration assay/product/Creatv MicroTech
Average 90 stars, based on 1 article reviews
cellsievetm microfiltration assay - by Bioz Stars, 2026-06
90/100 stars
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cellsieve tm  (Creatv MicroTech)
90
Creatv MicroTech cellsieve tm
Standardization and validation of CTC by IF×3 using breast, ovarian, and lung cancer cell lines: Patients’ blood samples spiked with titrating number (1000 cells, 750 cells, 375 cells, 250 cells/100 cells) of cell lines of different solid tumors using. Pictures were taken at 60× oil objective of an Olympus IX71 Microscope with DAPI/FITC/TRITC/CY5 filter sets. ( A ): MCF7 cells (750 cells/375 cells per 7.5 mL of patient’s blood) were used for spiking blood samples, and cells were captured on a microfilter and stained with a <t>CellSieve</t> enumeration kit (Creatv Microtech) with either DAPI/CK-FITC/EpCAM-PE/CD45-Cy5 ( Ai ) or DAPI/CK-FITC/CD31 PE/CD45-Cy5 ( Aii ). ( B ): OVCAR3 cells (100 cells per 7.5 mL of patient’s blood) were used for spiking blood samples, and cells were captured on a microfilter and stained with cell sieve enumeration kit (Creatv MicroTech) with DAPI/CK-FITC/EpCAM-PE/CD45-Cy5. ( C ): HCC1975 cells (1000 cells per 7.5 mL of patient’s blood) were used for spiking blood samples, and cells were captured on a microfilter and stained with cell sieve enumeration kit (Creatv Microtech) with DAPI/CK-FITC/EpCAM-PE/CD45-Cy5. ( D ): NCI-H441 cells (250 cells per 7.5 mL of patient’s blood) were used for spiking blood samples, and cells were captured on a microfilter and stained with cell sieve enumeration kit (Creatv Microtech) with DAPI/CK-FITC/EpCAM-PE/CD45-Cy5. The magnification, scale bar, and digital reticle are represented for each photomicrograph. Fluorescence images from DAPI, FITC, TRITC, and Cy5 channels were separated as pictures with a color bar. The fluorescence-photomicrographs presented the diameters (μm) of CTC and a representative WBC and their respective DAPI stained nucleus.
Cellsieve Tm, supplied by Creatv MicroTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cellsieve tm/product/Creatv MicroTech
Average 90 stars, based on 1 article reviews
cellsieve tm - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
ctchip fr1 microfluidic chips  (Biolidics Ltd)
90
Biolidics Ltd ctchip fr1 microfluidic chips
Standardization and validation of CTC by IF×3 using breast, ovarian, and lung cancer cell lines: Patients’ blood samples spiked with titrating number (1000 cells, 750 cells, 375 cells, 250 cells/100 cells) of cell lines of different solid tumors using. Pictures were taken at 60× oil objective of an Olympus IX71 Microscope with DAPI/FITC/TRITC/CY5 filter sets. ( A ): MCF7 cells (750 cells/375 cells per 7.5 mL of patient’s blood) were used for spiking blood samples, and cells were captured on a microfilter and stained with a <t>CellSieve</t> enumeration kit (Creatv Microtech) with either DAPI/CK-FITC/EpCAM-PE/CD45-Cy5 ( Ai ) or DAPI/CK-FITC/CD31 PE/CD45-Cy5 ( Aii ). ( B ): OVCAR3 cells (100 cells per 7.5 mL of patient’s blood) were used for spiking blood samples, and cells were captured on a microfilter and stained with cell sieve enumeration kit (Creatv MicroTech) with DAPI/CK-FITC/EpCAM-PE/CD45-Cy5. ( C ): HCC1975 cells (1000 cells per 7.5 mL of patient’s blood) were used for spiking blood samples, and cells were captured on a microfilter and stained with cell sieve enumeration kit (Creatv Microtech) with DAPI/CK-FITC/EpCAM-PE/CD45-Cy5. ( D ): NCI-H441 cells (250 cells per 7.5 mL of patient’s blood) were used for spiking blood samples, and cells were captured on a microfilter and stained with cell sieve enumeration kit (Creatv Microtech) with DAPI/CK-FITC/EpCAM-PE/CD45-Cy5. The magnification, scale bar, and digital reticle are represented for each photomicrograph. Fluorescence images from DAPI, FITC, TRITC, and Cy5 channels were separated as pictures with a color bar. The fluorescence-photomicrographs presented the diameters (μm) of CTC and a representative WBC and their respective DAPI stained nucleus.
Ctchip Fr1 Microfluidic Chips, supplied by Biolidics Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ctchip fr1 microfluidic chips/product/Biolidics Ltd
Average 90 stars, based on 1 article reviews
ctchip fr1 microfluidic chips - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier

Image Search Results


Studies on the clinical relevance of programmed death ligand 1-positive (PD-L1 + ) circulating tumor cells (CTCs) in non-small cell lung cancer (NSCLC) .

Journal: Cells

Article Title: Circulating Tumor Cell PD-L1 Expression as Biomarker for Therapeutic Efficacy of Immune Checkpoint Inhibition in NSCLC

doi: 10.3390/cells8080809

Figure Lengend Snippet: Studies on the clinical relevance of programmed death ligand 1-positive (PD-L1 + ) circulating tumor cells (CTCs) in non-small cell lung cancer (NSCLC) .

Article Snippet: Through a different size-based CTC-enrichment approach (CellSieve™ Microfiltration Assay, Creatv MicroTech) followed by immunostaining, Adams et al. [ ] investigated the expression of PD-L1 in different CTC subtypes, i.e., PDCTCs (prognostically relevant pathologically definable CTCs), EMTCTCs (CTCs undergoing epithelial-to-mesenchymal transition), and CAMLs (cancer-associated macrophage-like cells) in a prospective pilot study with 41 NSCLC patients (stage I–IV) undergoing radiotherapy, while 34% (14 of 41) received prior chemotherapy.

Techniques: Produced, Expressing

Standardization and validation of CTC by IF×3 using breast, ovarian, and lung cancer cell lines: Patients’ blood samples spiked with titrating number (1000 cells, 750 cells, 375 cells, 250 cells/100 cells) of cell lines of different solid tumors using. Pictures were taken at 60× oil objective of an Olympus IX71 Microscope with DAPI/FITC/TRITC/CY5 filter sets. ( A ): MCF7 cells (750 cells/375 cells per 7.5 mL of patient’s blood) were used for spiking blood samples, and cells were captured on a microfilter and stained with a CellSieve enumeration kit (Creatv Microtech) with either DAPI/CK-FITC/EpCAM-PE/CD45-Cy5 ( Ai ) or DAPI/CK-FITC/CD31 PE/CD45-Cy5 ( Aii ). ( B ): OVCAR3 cells (100 cells per 7.5 mL of patient’s blood) were used for spiking blood samples, and cells were captured on a microfilter and stained with cell sieve enumeration kit (Creatv MicroTech) with DAPI/CK-FITC/EpCAM-PE/CD45-Cy5. ( C ): HCC1975 cells (1000 cells per 7.5 mL of patient’s blood) were used for spiking blood samples, and cells were captured on a microfilter and stained with cell sieve enumeration kit (Creatv Microtech) with DAPI/CK-FITC/EpCAM-PE/CD45-Cy5. ( D ): NCI-H441 cells (250 cells per 7.5 mL of patient’s blood) were used for spiking blood samples, and cells were captured on a microfilter and stained with cell sieve enumeration kit (Creatv Microtech) with DAPI/CK-FITC/EpCAM-PE/CD45-Cy5. The magnification, scale bar, and digital reticle are represented for each photomicrograph. Fluorescence images from DAPI, FITC, TRITC, and Cy5 channels were separated as pictures with a color bar. The fluorescence-photomicrographs presented the diameters (μm) of CTC and a representative WBC and their respective DAPI stained nucleus.

Journal: Cancers

Article Title: A Laboratory-Friendly CTC Identification: Comparable Double-Immunocytochemistry with Triple-Immunofluorescence

doi: 10.3390/cancers14122871

Figure Lengend Snippet: Standardization and validation of CTC by IF×3 using breast, ovarian, and lung cancer cell lines: Patients’ blood samples spiked with titrating number (1000 cells, 750 cells, 375 cells, 250 cells/100 cells) of cell lines of different solid tumors using. Pictures were taken at 60× oil objective of an Olympus IX71 Microscope with DAPI/FITC/TRITC/CY5 filter sets. ( A ): MCF7 cells (750 cells/375 cells per 7.5 mL of patient’s blood) were used for spiking blood samples, and cells were captured on a microfilter and stained with a CellSieve enumeration kit (Creatv Microtech) with either DAPI/CK-FITC/EpCAM-PE/CD45-Cy5 ( Ai ) or DAPI/CK-FITC/CD31 PE/CD45-Cy5 ( Aii ). ( B ): OVCAR3 cells (100 cells per 7.5 mL of patient’s blood) were used for spiking blood samples, and cells were captured on a microfilter and stained with cell sieve enumeration kit (Creatv MicroTech) with DAPI/CK-FITC/EpCAM-PE/CD45-Cy5. ( C ): HCC1975 cells (1000 cells per 7.5 mL of patient’s blood) were used for spiking blood samples, and cells were captured on a microfilter and stained with cell sieve enumeration kit (Creatv Microtech) with DAPI/CK-FITC/EpCAM-PE/CD45-Cy5. ( D ): NCI-H441 cells (250 cells per 7.5 mL of patient’s blood) were used for spiking blood samples, and cells were captured on a microfilter and stained with cell sieve enumeration kit (Creatv Microtech) with DAPI/CK-FITC/EpCAM-PE/CD45-Cy5. The magnification, scale bar, and digital reticle are represented for each photomicrograph. Fluorescence images from DAPI, FITC, TRITC, and Cy5 channels were separated as pictures with a color bar. The fluorescence-photomicrographs presented the diameters (μm) of CTC and a representative WBC and their respective DAPI stained nucleus.

Article Snippet: The isolation and size-based enrichment of CTCs from blood was achieved by (CellSieve TM ; Creatv Microtech, Potomac, MD, USA) using precision, high-porosity lithographic microfilters (high capture efficiency precision CellSieve TM microfilters of biocompatible polymer with dense, uniform pores) [ , , ].

Techniques: Microscopy, Staining, Fluorescence